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1942 or 1943. "Gilead, Ohio (?), possibly Mount Gilead" is the uncertain caption. Although the photo does provide a few clues. 35mm Kodachrome transparency by John Vachon for the Farm Security Administration. View full size.
UPDATE: A couple of astute commenters have identified this as a building on Main Street in Mount Orab, Ohio. Google Street View gives a pretty good match. If there are any Shorpyite shutterbugs in Mount Orab, hie on over to the intersection of Main and High, send us some pics and help us lay to rest a mystery that's been bedeviling the Library of Congress for the past 65 years!